We have developed various technologies for chromatin genomics and genome editing. Click on the links for relevant publications, protocols and other resources:
DamID: a method for the mapping of in vivo protein-genome interactions.
m6A-tracer: a derivative of DamID, to visualize DNA that has contacted a protein of interest in living cells.
TRIP: a method to measure the impact of chromatin on gene regulation at thousands of genomic locations in parallel.
TIDE and TIDER: simple, cheap and quantitative methods to measure genome editing efficiency by CRISPR/Cas9.
SuRE: a massively parallel reporter assay to map and measure the activity of regulatory elements in entire mammalian genomes.
New tools under development
- A hybrid of DamID and CUT&RUN
- A method to keep genomic loci (such as enhancers and promoters) spatially separated inside the cell nucleus
- A variant of SuRE to test the functionality of thousands of enhancer/promoter pairs
- A variant of TRIP to test the impact of chromatin context on DNA repair
- A screen to find proteins that control gene regulation inside LADs
Contact us if you are interested!